Wyoming's only abortion clinic, Wellspring Health Access in Casper, began experiencing the direct impact of Gov. Mark Gordon's Human Heartbeat Act within days of its March 9 signing, with Wyoming Public Media reporting on March 17 that approximately a dozen patients had been turned away in the law's first week because cardiac activity had been detected in their pregnancies — typically around six weeks of gestation, often before women know they are pregnant. County 17, a Wyoming news outlet, reported on March 20 that Wellspring is "treating fewer patients while partial ban awaits court hearing," noting the clinic continues to provide services to patients below the six-week threshold as it simultaneously pursues a legal challenge to the law. Julie Burkhart, Wellspring's president, said the clinic is working with regional and national partners to help patients who can no longer obtain care in Wyoming access services out of state.

LifeNews, an anti-abortion news outlet, reported on March 19 that Wyoming's abortion ban was "already saving babies from abortions," framing the turned-away patients as evidence of the law achieving its intended purpose. Gov. Gordon celebrated the law's signing in a post on X: "Today I signed the Human Heartbeat Act into law, reaffirming my view that life is sacred. I resoundingly share the determination to defend the lives of unborn children and support the intentions behind the Human Heartbeat Act." Gordon added, however, that he was concerned the bill was "well-intentioned" but would face a "fragile legal effort with significant risk of ending in the courts rather than in lasting, durable policy" — an unusual expression of skepticism from the governor who signed the legislation.

Wyoming Public Media and ABC News reported the facts of the law's provisions: the Human Heartbeat Act prohibits abortion once cardiac activity is detected, with the only exception being a medical emergency threatening the mother's life or risking "serious or irreversible impairment of a major bodily function." There are no exceptions for pregnancies resulting from rape or incest — a provision that Gov. Gordon noted in his signing statement as a concern. Any person who intentionally violates the act is subject to a felony charge punishable by up to five years in prison and a ,000 fine. Wyoming is the fifth state to enact a heartbeat ban, following Florida, Georgia, Iowa, and South Carolina.

The ban is legally entangled with Wyoming's earlier abortion legislation. Wyoming passed a near-total abortion ban in 2022, but that law has been blocked by the Wyoming Supreme Court on state constitutional grounds. An earlier Wyoming law banning abortion pills was also struck down. The Human Heartbeat Act represents the legislature's third attempt to restrict abortion in Wyoming following the Dobbs ruling. Burkhart's legal challenge is expected to center on Wyoming's state constitution, which the Wyoming Supreme Court previously found contains an implied right to privacy that may protect abortion access — a state-law angle distinct from the federal constitutional framework that ended federal abortion rights in Dobbs.